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INTRODUCTION

A common operation in multiplexing is gridding proteins onto a substrate in an ordered array. Once arrayed, the substrate is probed with a fluorescent probe of interest, and is then analyzed to detect the locations where the probe bound to the substrate. An alternative to pin gridding, the most common means of achieving high-density arrays, is to use either BioJet Plus or Scienion for a non-contact dispense. Patented BioJet Plus technology involves the coupling of a microsolenoid valve to a high resolution syringe. The system is then synchronized with the XYZ motion allowing for very fast dispense with high accuracy and precision. Scienion dispensing is based on piezo technology that is also very fast. The Scienion technology dispenses from the picoliter range to the low nanoliter range, whileas the BioJet Plus technology dispenses from the low nanoliter range to the low microliter. Shown below are array examples achievable using either BioJet Plus or Scienion technology, each with decreasing center-to-center spacing from left to right.

array examples achievable using BioJet Plus technology, each with decreasing center-to-center spacing

Shown below are arrays of 10 x 10 drops, and a 1.0 mm center-to-center spacing (array density is 100 drops/cm2). The pattern was created in a two step process. The first step was dispensing sub-pattern A to each location, followed by dispensing the complementary sub-pattern B to each location.

arrays of 10 x 10 drops, and a 1.0 mm center-to-center spacing (array density is 100 drops/cm2)

Using BioJet Plus, non-contact arraying can be completed on glass slides, membranes, or in microtiter plates.


 

 
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