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A common operation in
genomics
research is gridding of genetic material onto a substrate in an ordered
array. Once arrayed, the substrate is probed with a fluorescently
labeled DNA probe of interest, and is then analyzed to detect the
locations where the probe bound to the substrate. An alternative to pin
gridding, the most common means of achieving high-density arrays, is to
use BioJet Plus for a non-contact dispense. Shown below are array
examples achievable using BioJet Plus technology, each with decreasing
center-to-center spacing from left to right.
Shown below are
arrays of 10 x 10
drops, and a 1.0 mm center-to-center spacing (array density is 100
drops/cm2). The pattern was created in a two step process. The first
step was dispensing sub-pattern A to each location, followed by
dispensing the complementary sub-pattern B to each location.
Using BioJet Plus,
non-contact arraying can be completed on glass slides, membranes, or in
microtiter plates.
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