Achieving High-Performance PCR at Low Reaction Volumes
BioDot’s latest application note demonstrates the capabilities of the NANOlab™-PCR platform for automated, nanoliter-scale PCR preparation, achieving 400 nL reaction volumes with dispensing CVs below 2.5% and amplification performance comparable to conventional 5 µL manual workflows. Leveraging non-contact dispensing, disposable-tip technology, and controlled environmental conditions, the system enables precise, reproducible reaction setup while minimizing contamination risk and reagent consumption.
This advancement addresses key challenges in PCR miniaturization, where dispensing accuracy, reaction consistency, and contamination control are critical to maintaining assay performance at reduced volumes. The study achieved comparable Cq values to manual controls (26.6 vs. 26.4), with no detectable amplification in no-template controls and greater than 90% reduction in reagent usage.
These results establish NANOlab-PCR as a scalable solution for laboratories developing molecular diagnostics, genomics, and other PCR-based applications where sample conservation, workflow automation, and operational efficiency are essential.
Download the application note to discover how BioDot is advancing PCR miniaturization through precision dispensing and automated liquid handling.
